rabbit igg anti mouse il 17a  (Santa Cruz Biotechnology)

Journal: PLOS ONE

Article Title: Effect of IL-17A on the immune response to pulmonary tuberculosis induced by high- and low-virulence strains of Mycobacterium bovis

doi: 10.1371/journal.pone.0307307

Figure Lengend Snippet: Primer sequences.

Article Snippet: Briefly, slides were incubated with rabbit IgG anti-mouse IL-17A (Santa Cruz Biotechnology, Santa Cruz, CA, USA; sc-7927) diluted 1:100 in the ImmunoDetector Protein Blocker/Antibody Diluent (Bio SB).

Techniques:

Journal: Nature communications

Article Title: Synovial microenvironment-influenced mast cells promote the progression of rheumatoid arthritis.

doi: 10.1038/s41467-023-44304-w

Figure Lengend Snippet: Fig. 6 | Prophylactic applications of cromolyn sodium and anti-IL-17A attenuated CIA. a The schematic diagram of study design: All mice received initial immunization on day 0 and booster immunization on day 21. After being randomly divided into 4 groups, mice were treated through intraperitoneal injection with the vehicle, cromolyn (25 mg/kg), IL-17A antibody (150 µg each time), or the combina- tion of the cromolyn and IL-17A antibody. Cromolyn and anti-IL-17A treatment was started from day 0 and day 21, respectively, and were both given every other day. b Arthritis scores were measured every day after booster immunization (n = 5 mice for each group, P = 0.0049 Vehicle vs Cromolyn, P = 0.0016 Vehicle vs Cromolyn +Anti-IL-17A, P = 0.0437 Anti-IL-17A vs Cromolyn+Anti-IL-17A). c Weight loss of each group showed as a percentage change observed once a week (n = 5 mice for each group, P = 0.0013 Vehicle vs Anti-IL-17A, P = 0.0165 Vehicle vs Cromolyn+Anti-IL- 17A, P = 0.0262 Cromolyn vs Anti-IL-17A). d Representative images of H&E and Safranin O-fast green staining of knee joints. Scale bar: 200 μm. e Statistical analysis

Article Snippet: Mice in the anti-IL-17A group received an intraperitoneal injection of 150 μg anti-mouse-IL-17A antibody (BioXcell) every other day fromday21.

Techniques: Injection, Staining

Journal: Experimental and Therapeutic Medicine

Article Title: Exosomes from Ub‑HBcAg‑overexpressing dendritic cells induce T‑lymphocyte differentiation and enhance cytotoxic T‑lymphocyte activity

doi: 10.3892/etm.2023.11866

Figure Lengend Snippet: Isolation and identification of Dexs-Ub-HBcAg. (A) Transmission electron microscopy of the mDexs ultrastructure. Scale bar, 200 nm. (B) Expression of the positive exosomal markers CD9, CD63 and TSG101, and of HBcAg was examined via the western blot analysis of Dexs lysates. (C) Size dispersion profile of mDexs evaluated using ZetaVIEW ® nanoparticle tracking analysis, indicating a size peak of 123.8 nm. Dexs, dendritic cell-derived exosomes; HBcAg, hepatitis B virus core antigen; Dexs-Ub-HBcAg, Dexs loaded with ubiquitinated HBcAg; Con, control; TSG101, tumor susceptibility gene 101.

Article Snippet: Subsequently, the membrane was blocked with 5% non-fat milk at room temperature for 1 h. The primary antibodies used were rabbit anti-mouse CD63 (1:500; cat. no. ab216130; Abcam), anti-CD9 (1:500; cat. no. ab92726; Abcam) and tumor susceptibility gene 101 (TSG101; 1:500; cat. no. 102286-T38; SinoBiological) monoclonal antibodies.

Techniques: Isolation, Transmission Assay, Electron Microscopy, Expressing, Western Blot, Dispersion, Derivative Assay, Virus, Control

Journal: The Journal of investigative dermatology

Article Title: Dermal Vγ6 + γδ T17 Cells Are Involved in Skin Pressure Ulcers in Mice.

doi: 10.1016/j.jid.2021.12.030

Figure Lengend Snippet: Figure 2. In vivo blockade of Vg6D gd T cell function and IL-17A reduced the infiltration of neutrophils in the skin and the size of scars. (a) The number of neutrophils and macrophages in the skin of male C57BL/6J mice during and after anti-Vg6 (1C10-1F7) or control antibody administration (n ¼ 3e4). (b) Representative images of the scar region on days 7, 10, and 14 after 1C10-1F7 mAbs treatment. Bar ¼ 500 mm. (c) Transition of the scar region after 1C10-1F7 mAbs or control antibody treatment (n ¼ 6e8). (d) The number of neutrophils in the skin of male C57BL/6J mice during and after antieIL-17A mAbs administration (n ¼ 3e4). (e) Representative images of scar region on days 7, 10, and 14 after antieIL-17A mAbs treatment. Bar ¼ 500 mm. (f) Transition of scar region after antieIL-17A mAbs or control antibody treatment (n ¼ 6). *P < 0.05; two-sample t-test. Ab, antibody; ip, intraperitoneal; IR, ischemiaereperfusion.

Article Snippet: In vivo treatment of mice with antiVg6 and antieIL-17A antibody Anti-mouse IL-17A antibody (clone 17F3) was obtained from Bioxcell (West Lebanon, NH).

Techniques: In Vivo, Cell Function Assay, Control

Journal: Scientific Reports

Article Title: Single-cell analysis reveals sexually dimorphic repertoires of Interferon-γ and IL-17A producing T cells in salivary glands of Sjögren’s syndrome mice

doi: 10.1038/s41598-017-12627-6

Figure Lengend Snippet: Frequency of IFN-γ and IL-17A producing T cells in the salivary glands of male and female mice.

Article Snippet: The nanowells were hybridized with the capture slide containing antibodies against IL-17A (AAM60, AbD Serotec) and IFN-γ (505827, Biolegend).

Techniques:

Journal: Scientific Reports

Article Title: Single-cell analysis reveals sexually dimorphic repertoires of Interferon-γ and IL-17A producing T cells in salivary glands of Sjögren’s syndrome mice

doi: 10.1038/s41598-017-12627-6

Figure Lengend Snippet: Microengraving shows greater infiltration by IL-17A and IFN-γ producing cells in the salivary glands of SjS S mice. Quantification of T lymphocytes isolated from the major salivary gland of B6 male (●), B6 female (○), B6.NOD- Aec1Aec2 male ( ), and B6.NOD- Aec1Aec2 female ( ) mice expressing ( a ) CD4 + IFN-γ + , ( b ) CD8 + IFN-γ + , ( c ) CD4 + IL-17A + , ( d ) CD8 + IL-17A + , ( e ) CD4 + IFN-γ + IL-17A + , or ( f ) CD8 + IFN-γ + IL-17A + . The frequency in percentage of was determined by using the percentage (multiplied by 100) of the total number of CD4 + IFN-γ+ (Th1), CD4 + IL-17A+ (Th17), CD8 + IFN-γ+ (Tc1), CD8 + IL-17A+ (Tc17), CD4 + IL-17A+IFN-γ+ (Th17/1), and CD8 + IL-17A+IFN-γ+ (Tc1/17) cells from wells with single live cells among the total number of wells with single CD4 + or CD8 + cells. Group comparisons were made using a Kruskall-Wallis test with Dunn’s post hoc test. Statistical significance was defined as a p value < 0.05. All results are presented as means ± s.e.m. Significance was determined as *p < 0.05, **p < 0.01.

Article Snippet: The nanowells were hybridized with the capture slide containing antibodies against IL-17A (AAM60, AbD Serotec) and IFN-γ (505827, Biolegend).

Techniques: Isolation, Expressing

Journal: Scientific Reports

Article Title: Single-cell analysis reveals sexually dimorphic repertoires of Interferon-γ and IL-17A producing T cells in salivary glands of Sjögren’s syndrome mice

doi: 10.1038/s41598-017-12627-6

Figure Lengend Snippet: MEME analysis of high frequency CDR3 regions from SjS S mice. Amino acid motif analysis was performed on the high frequency CDR3 sequences from the B6.NOD- Aec1/2 mice. Bit height corresponds to the likelihood of the amino acid in each position. Blue – hydrophobic, neutral amino acids, Red – positively charge hydrophilic amino acids, Green – Neutral hydrophilic amino acids, Magenta – negatively charged hydrophilic amino acids, Orange-glycine, Teal-Tyrosine, Pink-Histidine (positively charged moderately hydrophobic). E-value indicates the model confidence for the amino acid in that position. Cytokine indicates the secreted molecule(s) associated with the motif, V/J indicates recombination gene segment pairings, and % pop. Indicates the percent of the motif present in the IFN-γ or the IL-17A producing population. *Most popular V/J combination. **Motif located in both IL-17A and IFN-γ producing populations in both TCRα and TCRβ chains.

Article Snippet: The nanowells were hybridized with the capture slide containing antibodies against IL-17A (AAM60, AbD Serotec) and IFN-γ (505827, Biolegend).

Techniques: